![]() Flush the extracorporeal blood access line by withdrawing 5 mL of blood into a syringe and discard the syringe.ī. For extracorporeal line testing for ACT:Ī.Withdraw the sample into a fresh plastic syringe with no anticoagulant, and test immediately. Withdraw 2 mL of blood into a syringe and discard it.Ĭ. Fluid drip through the line must be discontinued.ī. If a second measurement is needed, draw a fresh sample.Ī.For venipuncture, some experts recommend drawing and discardingĪ sample of at least 1 mL prior to drawing samples for coagulation testing.Use plain plastic syringes or plastic evacuated tubes with no anticoagulant, activators, or serum separators.The ACT test can be performed using venous or arterial samples, while the PT/INR test can be performed using capillary or venous samples.Do not “milk” finger or heel while collecting sample.Allow alcohol to dry over puncture site before collecting sample.For all analytes including ionized calcium, use plain or balanced heparin capillary tubes. Skin puncture: Lithium heparin capillary tubes for testing all analytes but ionized calcium. Do not leave tourniquet on for more than 2 minutes. Venous: Collection tube with lithium or sodium heparin filled to capacity and mixed by gentle inversion at least 10 times. If not tested immediately, remix and discard 2 drops of blood before filling cartridge.Avoid drawing air into syringes for blood gas and ionized calcium tests.Test for other analytes within 30 minutes. Samples for pH, PCO 2, PO 2, TCO 2 and ionized calcium should be tested within 10 minutes. Mix heparinized syringes by rolling between palms for at leastĥ seconds in 2 directions, then invert the syringe repeatedly for at least 5 For ionized calcium, use balanced heparin syringes. Arterial: Plain syringe, heparinized syringe labeled for analytes to be tested and filled to capacity, or syringe with minimum volume of heparin to prevent clotting (10 U/mL of blood).
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